WebHT BioFlex® culture plates are 14 mm 24-well plates with flexible silicone elastomer well bottoms with a total growth surface area of 37.47 cm2 (1.56 cm2/well; membrane … WebJan 1, 2013 · After harvesting the aggregates from Aggrewell plates, they are added to the prepared Bioflex plate wells containing the inactivated feeder cells and left for 2–3 h at room temperature to aid their adherence to the surface, before being placed in a 32°C, 5% CO 2 incubator for 24 h (see Note 12). 3. The next morning, the Flexcell system is ...
Cessation of cyclic stretch induces atrophy of C2C12 myotubes
WebJan 1, 2005 · For tensile loading, HIG-82 cells were seeded on the central area of collagen type I coated Bioflex® 6-well cell culture plates (Randolph, NJ, USA) corresponding to the area of uniform strain for ... WebWith high stretch treatment, cells grown on Bioflex plates were exposed to a 13% cyclic strain at 0.5 Hz (1 s of deformation alternating with 1 s of relaxation, Flexcell 5000, FlexCell International, Hillsborough, NC, USA) to simulate the strain during MV for 72 h. Without high stretch treatment, cells grown in Bioflex plates were maintained in ... how he fell in love 123movies
Using 3D Culture to Investigate the Role of Mechanical ... - Springer
WebFlexcell International Corp flexible bottom bioflex plates Flexible Bottom Bioflex Plates, supplied by Flexcell International Corp, used in various techniques. Bioz Stars score: … WebMay 3, 2013 · We tested a cell culture model of disuse atrophy by the cessation of repetitive bouts of cyclic stretch in differentiated C2C12 myotubes. Myotubes were subjected to cyclic strain (12%, 0.7 Hz, 1 h/d) on collagen-I-coated Bioflex plates using a computer-controlled vacuum stretch apparatus (Flexcell Int.) for 2 (2dSTR) or 5 (5dSTR) consecutive days. WebBioflex Nutrition, 7 Crabtree Road, Grove, Tasmania, 7109. ABN 17 158 981 447. Information. About Products Contact Terms & Conditions ... how he fell in love 2015 watch online