Bt2a bt2b primers

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August undefined, 2024

WebPrimer sequence: Primer concentration: Amplification of long PCR products Optimizing cycling conditions Optimizing PCR additives Optimizing 3' to 5' exonuclease activity Enzymes used in PCR Taq DNA polymerase Hot-start DNA polymerase High-fidelity DNA polymerase PCR cycling Commonly used terms in PCR PCR quantification WebMA). The primers were Bt2a (forward): 5¢-GGTAACCAAATCGG TGCTGCTTTC-3¢, and Bt2b (reverse): 5¢-ACCCTCAGTGTAGT GACCCTTGGC …

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http://lutzonilab.org/beta-tubulin/ Web黄宏强，范小燕，陶亚群，鲁红学，邓建新 (长江大学农学院，湖北，荆州 434025) 三七黑斑病病原菌的复核鉴定 combing natural hair

PCR-RFLP on β-tubulin gene for rapid identification of the …

WebJun 27, 2024 · To confirm the species diagnosis, the structure of species-specific DNA loci were studied: region ITS1–5.8S-ITS2 (primers ITS5 / ITS4), fragments of β-tubulin (Bt2a / Bt2b) and TEF 1α (EF1-728F / EF1-986R) genes. DNA isolation, extraction of amplicons, sequencing and data processing were performed as described in Kutuzova et al. . WebNucleic acid sequence analysis is an extensively applied method in Genomics studies. Universal primers are complementary to nucleotide sequences that are very common in a particular set of DNA molecules and cloning vectors. Thus, they are able to bind to a wide variety of DNA templates. During sequencing, primers are annealed to the denatured ... WebNov 7, 2024 · The amplicons of β-tubulin gene (primers Bt2a/Bt2b) were digested with Alw I (BspPI) enzyme (Thermo Scientiﬁc, USA) for 4 h at 55 °C and heat-terminated at 80 °C for 20 min. The reaction mixture … drugs for gout and hyperuricemia

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WebMar 23, 2013 · Partial sequences of the beta-tubulin gene were determined by using the primers Bt2a and Bt2b for PCR amplification and sequencing and underwent a BLAST search at the National Centre for Biotechnology Information, these results confirmed that the isolated fungus was P. digitatum. Conclusion WebMay 16, 2024 · were sequenced using primers ITS1/ITS4, EF1-728F/EF1-986R and BT2a/BT2b, and the sequences were deposited . in GenBank with accession numbers MZ028464 (ITS), MW862012 (TEF1-α) and MZ028463 (tub2 ... combining 2 bee hivesWebUsing the primer setBt2aandBt2b,a362-bpfragmentwasamplifiedfromP.striiformis f. sp. tritici, while a 496-bp fragment was amplified from both P.triticinaandP.graminisf.sp.tritici (Fig.1).TheDNAfragments of P. triticina and P. graminis f. sp. tritici shared 100% homology, whereas the specific DNAfragment of P. striiformis f.sp.tritici shared … drugs for gastritis treatment

"WebAug 16, 2024 · For molecular identification the internal transcribed spacer of rDNA region (ITS), the genes of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT) and β-tubulin (TUB), and Apn2-Mat1-2 intergenic spacer (ApMat) were sequenced using ITS1/ITS4, GDF/GDR, ACT-512F/ACT-783R, Bt2a/Bt2b, CgDL-F6/CgMAT1-F2 … " - Bt2a bt2b primers

Bt2a bt2b primers

WebInterchangeable Core Lock Pin, Best, Top, #2, 0.108 Diameter x 0.025 Length, Brass, For A2 System, 100 each per Bag WebFor molecular identification, DNA was extracted, and PCRs were performed with primers ITS1/ITS4 for the ITS region (White et al. 1990), primers Bt2a/Bt2b for the β-tubulin gene (TUB) (Glass and Donaldson 1995), and primers EF1-728F/EF1-986R for the translation elongation factor 1-alpha gene (EF1-α) (Carbone and Kohn 1999).

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WebWe developed and validated a discriminative low-cost PCR-based test to discriminate Aspergillus isolates at the species level. The Beta tubulin gene of various reference … WebThe interest of Universal primers. Nucleic acid sequence analysis is an extensively applied method in Genomics studies. Universal primers are complementary to nucleotide …

WebFeb 8, 2024 · The internal transcribed spacer (ITS) region of rDNA, transcription elongation factor (tef-1α) and β-tubulin (tub2) gene portions were sequenced using ITS1/ITS4, EF1 … WebOct 28, 2024 · For β-tubulin gene, TU1/TU2 primers was performed by initial denaturation at 95 °C for 4 min, followed by 35 cycles of 95 °C for 30 s, 55 °C for 30 s, 72 °C for 1 …

WebJul 25, 2024 · For molecular identification, DNA was extracted and the internal transcribed spacer (ITS) region of ribosomal DNA and portions of the β-tubulin and elongation factor 1 alpha (EF1-α) genes of a selected isolate (Gc_01) were amplified using primers ITS1/ITS4 (White et al., 1990), Bt2a/Bt2b (Glass & Donaldson, 1995) and EF1-728F/EF1-1199R … Webstandard amplification reactions and the following primer pair: Bt2a (59 GGT AAC CAA ATC GGT GCT TTC 39) and Bt2b (59 ACC CTC AGT GTA GTG ACC CTT GGC 39), as described by Glass and Donaldson [10]. Part of the calmodulin gene region was amplified using the cmd5 (59 CCG AGT ACA AGG AGG Table 1. Aspergillus bertholletius isolates …

WebJan 11, 2024 · To confirm the morphological identification, the internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α) and β-tubulin genes of the representative isolate Wn-4 were sequenced using the primers ITS1/4, EF1-728 F/986R and Bt2a/Bt2b.

WebNov 10, 2024 · In addition, the β-tubulin and elongation factor 1-α targets were amplified by primers Bt2a / Bt2b (Glass and Donaldson, 1995) and EF1–728 F / EF1-986R (Carbone and Kohn, 1999), respectively,... drugs for hashimoto\u0027s diseaseWebJul 24, 2024 · The internal transcribed spacer (ITS) region (ITS1/ITS4 primers), β-tubulin (Bt2a/Bt2b primers), and calmodulin gene (Cmd5/Cmd6 primers) were amplified using polymerase chain reaction (PCR) ( Henry et al. 2000; Yaguchi et al. 2007 ). The PCR products were purified and sequenced. drugs for dry mouth combining 2 cells of text in excelWebForward: Bt2a 5’-GGTAACCAAATCGGTGCTGCTTTC. Reverse: Bt2b 5’-ACCCTCAGTGTAGTGACCCTTGGC. Annealing temperature: 54°C. These primers … drugs for gerd and peptic ulcer diseaseWebMay 16, 2024 · The ITS, EF1-α and tub2 gene regions of the reference isolate (Code: WL2-D) were sequenced using primers ITS1/ITS4, EF1-728F/EF1-986R and BT2a/BT2b, and the sequences were deposited in GenBank with accession numbers MZ028464 (ITS), MW862012 (TEF1-α) and MZ028463 (tub2). drugs for hair regrowthWebThe primer and Mg2+ concentration in the PCR buffer and annealing temperature of the reaction may need to be optimized for each primer pair for efficient PCR. In addition, … drugs for gonorrhea treatmentWebapplied. The amplicons of β-tubulin gene (primers Bt2a/Bt2b) were digested with AlwI (BspPI) enzyme (Thermo Scientiﬁ c, USA) for 4 h at 55 °C and heat-terminated at 80 °C … drugs for gram positive cocci