Hifi pcr酶

WebAccuStart Taq DNA Polymerase HiFi is a robust alternative PCR enzyme for both routine PCR applications as well as amplification of problematic templates. Components AccuStart Taq DNA polymerase HiFi 5 units/µL in 50% glycerol, 20 mM Tris-HCl, 40 mM NaCl, 0.1 mM EDTA, and stabilizers. HiFi PCR Buffer (10X) 600 mM Tris-SO 4 (pH 8.9), 180 mM … WebGDP-HiFi is a new recombinant enzyme with genetic modification for its amino acid sequence, which results 70 times better fidelity than Taq DNA polymerase and an extremely fast elongation rate (as fast as 15 seconds per kilo base, kb).GDP-HiFi has higher stability at high temperature, and this property makes GDP-HiFi perform very well for GC-rich PCR.

2×TransTaq® High Fidelity (HiFi) PCR SuperMix I/II - TransGen …

Web639298. CloneAmp ™ HiFi PCR Premix. 200 Rxns. ¥2,468. 收藏产品 加入购物车. CloneAmp HiFi PCR Premix是一种方便的2X预混合物,可提供高保真和高扩增效率的DNA扩增,是In-Fusion PCR克隆的理想选择。. 它包含dNTP和优化的缓冲液,可进行快速PCR反应以及高通量反应。. WebM5 Taq HiFi DNA 聚合酶比普通Taq 酶扩增效率高、错配率低,具有快速简便、灵敏度高、特异性强、稳定性好等优点。 可最大限度 地减少人为误差,可用于高特异性PCR 反应 … highcraft uk https://placeofhopes.org

KAPA HiFi高保真酶及Ready Mix - 云生物

Web23 de fev. de 2024 · In the ReadyMix PCR Kit, the enzyme is supplied in a convenient 2X ReadyMix format, containing all reaction components except primers and template. The ReadyMix contains KAPA HiFi HotStart DNA … WebTurboQ 即時定量聚合酶連鎖反應儀,專為多重PCR和常規即時定量PCR核酸檢測設計,同一樣本最多可同時檢測四種核酸序列。產品承繼藍光生技十多年的PCR開發核心技術能 … WebManufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq. Robust reactions - maximal success with minimal optimization. Offered with multiple buffers for customized reaction setup for different DNA templates. high craggy hill crossword clue

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Category:UCP HiFidelity PCR Kit - Qiagen

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Hifi pcr酶

CiBER-seq,让基因调控网络研究更简单 - 知乎

Web合理选择耐热DNA聚合酶是PCR成败与否的一个关键因素。. 选择最合适的耐热聚合酶,是进行PCR实验首先要考虑的问题。. 许多耐热DNA聚合酶的主要区别在于 特异性、保真性、耐热性、扩增速率、扩增片段长度 等几个指标。. 耐高温的DNA聚合酶分为三类。. 第一类 ... WebM5 Taq HiFi DNA 聚合酶比普通Taq 酶扩增效率高、错配率低,具有快速简便、灵敏度高、特异性强、稳定性好等优点。 可最大限度 地减少人为误差,可用于高特异性PCR 反应及GC 含量较高(>60%)具有二级结构等复杂模板的扩增和大规模基因检测。

Hifi pcr酶

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Webhifi 热启动高保真dna聚合酶应用. 用于常规测序的pcr(直接测序或克隆pcr产物的测序) 新一代测序文库扩增(与kapa文库扩增方案配合使用时) 扩增片段dna用于克隆和蛋白质表达或基因组表征. 定点突变 . kapa hifi dna聚合酶扩增特点. 高dna亲和力,无需辅助蛋白 … WebAt Roche we deliver a broad and growing selection of advanced research tools that accelerate life science discovery. Committed to innovation, quality, and service, we offer an extensive product portfolio featuring cutting-edge instruments and reagents for genome sequencing, microarray analysis, nucleic acid purification, real-time PCR, and cell ...

WebCloneAmp™ HiFi PCR Premix Protocol-At-A-Glance (092612) www.clontech.com Clontech Laboratories, Inc. A Takara Bio Company Page 1 of 2 CloneAmp HiFi PCR Premix (Cat. … WebManufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X …

WebPCR Characteristics of Proofreading DNA Polymerases The source, composition, and PCR characteristics of commercial proofreading enzymes are provided in Table 1. PfuUltra and PfuUltra II (fusion) DNA polymerases are formulated with a proprietary Pfu mutant that provides 3-fold higher fidelity than Pfu. In addition, the PfuTurbo and PfuUltra Web大肠杆菌37 ℃有氧培养,液体培养时摇床转速200 r/min;筛选半乳糖苷酶(β-galactosidase,LacZ)突变株时,固体培养基(琼脂糖浓度1.5 %)中加入终浓度500 mmol/L 5-溴-4-氯-3-吲哚-β -半乳糖苷(X-gal)和异丙基-β-D-硫代半乳糖苷以建立蓝白斑筛选体系。

WebMCLAB Products. I-5™ 2X Hi-Fi PCR Master Mix. I-5 High-Fidelity DNA Polymerase is an ultra-high fidelity and high processivity enzyme. It produces the most accurate copies of …

WebManufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq. Robust reactions - … high crags schoolWebAre you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection … how fast can light travel mphWeb丁香通为您找到830条PCR中氯化镁和taq酶信息,包括PCR中氯化镁和taq酶报价行情,优质供应商,图片,品牌等最新信息,丁香通为买家提供用户服务,诚信保障等服务,批发采购PCR中氯化镁和taq酶,上丁香通移动站。 how fast can lava flow in kmWebRoche罗氏荧光定量试剂4913914001, 产品描述 Components 2x浓度的FastStart Universal SYBR Green Master (Rox)预混液含有FastStart Taq DNA聚合酶、反应缓冲液、核苷酸(dATP, dCTP, dGTP, dUTP)、SYBR Green I以及参考染料。 Qual highcraig quarry johnstoneWeb了解pcr的历史、pcr 反应配制考虑因素、dna聚合酶选择的重要性、常见pcr方法和应用以及疑难解答。 T m 计算器 使用我们的应用程序计算引物的 T m 值并评估使用不同DNA聚合酶时合适的退火温度。 high crags school shipleyWeb13 de dez. de 2013 · We generally recommend using Q5 High-Fidelity DNA Polymerase at a final concentration of 20 units/ml (1.0 unit/50 μl reaction). However, the optimal concentration of Q5 High-Fidelity DNA Polymerase may vary from 10–40 units/ml (0.5–2 units/50 μl reaction) depending on amplicon length and difficulty. Do not exceed 2 … high crag buttermerehigh cranial vault