WebMay 15, 2024 · In this study, a rapid and sensitive hydrophilic interaction liquid chromatography-tandem mass spectrometry method was established and validated for the quantification of L-theanine in juvenile rat plasma and tissues. Chromatographic separation was conducted via an Agilent ZORBAX HILIC plus column in gradient elution mode. WebAgilent ZORBAX HILIC Plus columns are for use in hydrophilic interaction chromatography (HILIC) applications, typically used for retention and resolution of small polar compounds. …
Association between Organophosphate Ester Exposure and …
WebOct 29, 2009 · HILIC versus normal phase chromatography. I want to separate/detect the broadest range of hydrophilic compounds in biological matrices (mainly urine and plasma) and use mass spectrometry for detection. It's ment as a kind of metabolomics approach. I am thinking of buying a Zorbax HILIC plus or a Zorbax Rx SIL (normal phase) column to … WebBecause the separation mechanism in HILIC methods involves the adsorption of a water layer on the particle surface, it is very important to completely reestablish or “reset” this water layer in between injections to ensure retention time reproducibility. We recommend equilibration with a minimum of 10 column volumes starting when the ... opening group home in texas
HILIC Method Development Thermo Fisher Scientific - US
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